The Eppendorf Multiporator uses soft pulse technology to apply extremely short electric pulses for the highest survival rates, ensuring cell-damaging influences are minimised. Electroporation is the use of short, intensive current surges (pulses) to generate reversible openings (pores) in a membrane. This allows for the introduction of foreign molecules into cells. The hyperosmolar buffer system swells the cell for easier membrane penetration, leading to higher transfection rates.
The relevant parameters and voltage duration are directly set, and the patented electronic pulse discharge ensures that they will be maintained exactly, independent of the sample resistance, producing reliable and reproducible results. Applications of the Multiporator include, transfection of animal and human cell lines, embryonic stem cells, primary cells, oocytes and plant cells. This is because the product is useful for efficient and gentle electroporation of gram-positive and gram-negative bacteria and yeast in addition to eukaryotic cells
Features of the Eppendorf Multiporator :
- Patented microprocessor- based pulse discharge.
- Compact unit that is small and portable.
- Soft Pulse technology – ensures cell damaging influences are minimised.
- Electroporation of gram-positive and gram-negative bacteria and yeast, in addition to eukaryotic cells.
- Product is easy to disinfect.
- Pulse voltage is 5V to 300V.
- Unit provides from 1 to 99 multiple pulses in 1 s intervals.
- Include an RS-232 interface for data printout.